Articles

Transcription factor signal transducer and activator of transcription 6 (STAT6) is an inhibitory factor for adult myogenesis

The signal transducer and activator of transcription 6 (STAT6) transcription factor plays a vitally important role in immune cells, where it is activated mainly by interleukin-4 (IL-4). Because IL-4 is an esse...

Authors: Mitsutoshi Kurosaka, Yuji Ogura, Shuichi Sato, Kazuhisa Kohda and Toshiya Funabashi

Single cell RNA-seq analysis of the flexor digitorum brevis mouse myofibers

Skeletal muscle myofibers can be separated into functionally distinct cell types that differ in gene and protein expression. Current single cell expression data is generally based upon single nucleus RNA, rath...

Authors: Rohan X. Verma, Suraj Kannan, Brian L. Lin, Katherine M. Fomchenko, Tim O. Nieuwenhuis, Arun H. Patil, Clarisse Lukban, Xiaoping Yang, Karen Fox-Talbot, Matthew N. McCall, Chulan Kwon, David A. Kass, Avi Z. Rosenberg and Marc K. Halushka

Nanopattern surface improves cultured human myotube maturation

In vitro maturation of human primary myoblasts using 2D culture remains a challenging process and leads to immature fibers with poor internal organization and function. This would however represent a valuable sys...

Authors: Jessica Brunetti, Stéphane Koenig, Arthur Monnier and Maud Frieden

Multi-omics reveals age-related differences in the diaphragm response to mechanical ventilation: a pilot study

Old age is associated with a significantly increased mortality in COVID-19 patients exposed to long-term controlled mechanical ventilation (CMV) and suggested to be due to the hyperinflammatory response associ...

Authors: Qiong Lyu, Ya Wen, Xiang Zhang, Alex B. Addinsall, Nicola Cacciani and Lars Larsson

Severe acute myopathy following SARS-CoV-2 infection: a case report and review of recent literature

SARS-CoV2 virus could be potentially myopathic. Serum creatinine phosphokinase (CPK) is frequently found elevated in severe SARS-CoV2 infection, which indicates skeletal muscle damage precipitating limb weakne...

Authors: Badrul Islam, Mohiuddin Ahmed, Zhahirul Islam and S. M. Begum

Muscle atrophy induced by overexpression of ALAS2 is related to muscle mitochondrial dysfunction

ALAS2 (delta-aminolevulinate synthase 2) is one of the two isoenzymes catalyzing the synthesis of delta-aminolevulinic acid (ALA), which is the first precursor of heme synthesis. ALAS2-overexpressing transgeni...

Authors: Yahui Peng, Jihong Li, Dixian Luo, Shuai Zhang, Sijia Li, Dayong Wang, Xidi Wang, Zhujun Zhang, Xue Wang, Changhui Sun, Xu Gao, Yang Hui and Rongzhang He

Retraction Note to: Exercise-induced mitochondrial p53 repairs mtDNA mutations in mutator mice

An amendment to this paper has been published and can be accessed via the original article.

Authors: Adeel Safdar, Konstantin Khrapko, James M. Flynn, Ayesha Saleem, Michael De Lisio, Adam P. W. Johnston, Yevgenya Kratysberg, Imtiaz A. Samjoo, Yu Kitaoka, Daniel I. Ogborn, Jonathan P. Little, Sandeep Raha, Gianni Parise, Mahmood Akhtar, Bart P. Hettinga, Glenn C. Rowe…

A novel approach for the isolation and long-term expansion of pure satellite cells based on ice-cold treatment

Satellite cells (SCs) are muscle stem cells capable of regenerating injured muscle. The study of their functional potential depends on the availability of methods for the isolation and expansion of pure SCs wi...

Authors: Anna Benedetti, Gianluca Cera, Daniele De Meo, Ciro Villani, Marina Bouche and Biliana Lozanoska-Ochser

MiR-1290 promotes myoblast differentiation and protects against myotube atrophy via Akt/p70/FoxO3 pathway regulation

Sarcopenia is a common skeletal disease related to myogenic disorders and muscle atrophy. Current clinical management has limited effectiveness. We sought to investigate the role of miR-1290 in myoblast differ...

Authors: Ji Che, Cuidi Xu, Yuanyuan Wu, Peiyu Jia, Qi Han, Yantao Ma, Xiaolei Wang and Yongjun Zheng

Dynamics of myogenic differentiation using a novel Myogenin knock-in reporter mouse

Myogenin is a transcription factor that is expressed during terminal myoblast differentiation in embryonic development and adult muscle regeneration. Investigation of this cell state transition has been hampered ...

Authors: Maria Benavente-Diaz, Glenda Comai, Daniela Di Girolamo, Francina Langa and Shahragim Tajbakhsh

Epigenetic regulation of satellite cell fate during skeletal muscle regeneration

In response to muscle injury, muscle stem cells integrate environmental cues in the damaged tissue to mediate regeneration. These environmental cues are tightly regulated to ensure expansion of muscle stem cel...

Authors: Jimmy Massenet, Edward Gardner, Bénédicte Chazaud and F. Jeffrey Dilworth

Muscle spindle function in healthy and diseased muscle

Almost every muscle contains muscle spindles. These delicate sensory receptors inform the central nervous system (CNS) about changes in the length of individual muscles and the speed of stretching. With this i...

Authors: Stephan Kröger and Bridgette Watkins

Sarcoglycan A mutation in miniature dachshund dogs causes limb-girdle muscular dystrophy 2D

A cohort of related miniature dachshund dogs with exercise intolerance, stiff gait, dysphagia, myoglobinuria, and markedly elevated serum creatine kinase activities were identified.

Authors: James R. Mickelson, Katie M. Minor, Ling T. Guo, Steven G. Friedenberg, Jonah N. Cullen, Amanda Ciavarella, Lydia E. Hambrook, Karen M. Brenner, Sarah E. Helmond, Stanley L. Marks and G. Diane Shelton

Analysis of human satellite cell dynamics on cultured adult skeletal muscle myofibers

Maintaining stem cells in physiologically relevant states is necessary to understand cell and context-specific signalling paradigms and to understand complex interfaces between cells in situ. Understanding hum...

Authors: Peter Feige, Eve C. Tsai and Michael A. Rudnicki

Mitochondrial dysfunction and consequences in calpain-3-deficient muscle

Nonsense or loss-of-function mutations in the non-lysosomal cysteine protease calpain-3 result in limb-girdle muscular dystrophy type 2A (LGMD2A). While calpain-3 is implicated in muscle cell differentiation, ...

Authors: Vanessa E. Jahnke, Jennifer M. Peterson, Jack H. Van Der Meulen, Jessica Boehler, Kitipong Uaesoontrachoon, Helen K. Johnston, Aurelia Defour, Aditi Phadke, Qing Yu, Jyoti K. Jaiswal and Kanneboyina Nagaraju

Meeting report: the 2020 FSHD International Research Congress

Authors: Michael Kyba, Robert J. Bloch, Julie Dumonceaux, Scott Q. Harper, Silvère M. van der Maarel, Francis M. Sverdrup, Kathryn R. Wagner, Baziel van Engelen and Yi-Wen Chen

The role of Nrf2 in acute and chronic muscle injury

The nuclear factor erythroid 2-related factor 2 (Nrf2) is considered as a master cytoprotective factor regulating the expression of genes encoding anti-oxidant, anti-inflammatory, and detoxifying proteins. The...

Authors: Iwona Bronisz-Budzyńska, Magdalena Kozakowska, Paulina Podkalicka, Neli Kachamakova-Trojanowska, Agnieszka Łoboda and Józef Dulak

Tetraspanin CD82 is necessary for muscle stem cell activation and supports dystrophic muscle function

Tetraspanins are a family of proteins known to assemble protein complexes at the cell membrane. They are thought to play diverse cellular functions in tissues by modifying protein-binding partners, thus bringi...

Authors: Arielle Hall, Tatiana Fontelonga, Alec Wright, Katlynn Bugda Gwilt, Jeffrey Widrick, Alessandra Pasut, Francesco Villa, Cynthia K. Miranti, Devin Gibbs, Evan Jiang, Hui Meng, Michael W. Lawlor and Emanuela Gussoni

MyoSight—semi-automated image analysis of skeletal muscle cross sections

Manual analysis of cross-sectional area, fiber-type distribution, and total and centralized nuclei in skeletal muscle cross sections is tedious and time consuming, necessitating an accurate, automated method o...

Authors: Lyle W. Babcock, Amy D. Hanna, Nadia H. Agha and Susan L. Hamilton

Ryanodine receptor 1-related disorders: an historical perspective and proposal for a unified nomenclature

The RYR1 gene, which encodes the sarcoplasmic reticulum calcium release channel or type 1 ryanodine receptor (RyR1) of skeletal muscle, was sequenced in 1988 and RYR1 variations that impair calcium homeostasis an...

Authors: Tokunbor A. Lawal, Joshua J. Todd, Jessica W. Witherspoon, Carsten G. Bönnemann, James J. Dowling, Susan L. Hamilton, Katherine G. Meilleur and Robert T. Dirksen

In remembrance of David Yaffe

Authors: Margaret Buckingham and Eldad Tzahor

Complementary NAD⁺ replacement strategies fail to functionally protect dystrophin-deficient muscle

Duchenne muscular dystrophy (DMD) is a progressive muscle wasting disorder stemming from a loss of functional dystrophin. Current therapeutic options for DMD are limited, as small molecule modalities remain la...

Authors: David W. Frederick, Alan V. McDougal, Melisa Semenas, Johanna Vappiani, Andrea Nuzzo, John C. Ulrich, J. David Becherer, Frank Preugschat, Eugene L. Stewart, Daniel C. Sévin and H. Fritz Kramer

A novel chemical-combination screen in zebrafish identifies epigenetic small molecule candidates for the treatment of Duchenne muscular dystrophy

Duchenne muscular dystrophy (DMD) is a severe neuromuscular disorder and is one of the most common muscular dystrophies. There are currently few effective therapies to treat the disease, although many small-mo...

Authors: Gist H. Farr III, Melanie Morris, Arianna Gomez, Thao Pham, Elisabeth Kilroy, Elizabeth U. Parker, Shery Said, Clarissa Henry and Lisa Maves

Pro-myogenic small molecules revealed by a chemical screen on primary muscle stem cells

Satellite cells are the canonical muscle stem cells that regenerate damaged skeletal muscle. Loss of function of these cells has been linked to reduced muscle repair capacity and compromised muscle health in a...

Authors: Sean M. Buchanan, Feodor D. Price, Alessandra Castiglioni, Amanda Wagner Gee, Joel Schneider, Mark N. Matyas, Monica Hayhurst, Mohammadsharif Tabebordbar, Amy J. Wagers and Lee L. Rubin

Dnmt3b regulates DUX4 expression in a tissue-dependent manner in transgenic D4Z4 mice

Facioscapulohumeral muscular dystrophy (FSHD) is a skeletal muscle disorder that is caused by derepression of the transcription factor DUX4 in skeletal muscle cells. Apart from SMCHD1, DNMT3B was recently iden...

Authors: Linde F. Bouwman, Bianca den Hamer, Elwin P. Verveer, Lente J. S. Lerink, Yvonne D. Krom, Silvère M. van der Maarel and Jessica C. de Greef

High-throughput screening identifies modulators of sarcospan that stabilize muscle cells and exhibit activity in the mouse model of Duchenne muscular dystrophy

Duchenne muscular dystrophy (DMD) is a degenerative muscle disease caused by mutations in the dystrophin gene. Loss of dystrophin prevents the formation of a critical connection between the muscle cell membran...

Authors: Cynthia Shu, Liubov Parfenova, Ekaterina Mokhonova, Judd R. Collado, Robert Damoiseaux, Jesus Campagna, Varghese John and Rachelle H. Crosbie

Exercise enhances mitochondrial fission and mitophagy to improve myopathy following critical limb ischemia in elderly mice via the PGC1a/FNDC5/irisin pathway

Elderly populations are susceptible to critical limb ischemia (CLI), but conventional treatments cannot significantly decrease amputation and mortality. Although exercise is an effective “non-pharmacological m...

Authors: Wuyang He, Peng Wang, Qingwei Chen and Chunqiu Li

Drosophila myosin mutants model the disparate severity of type 1 and type 2B distal arthrogryposis and indicate an enhanced actin affinity mechanism

Distal arthrogryposis (DA) is a group of autosomal dominant skeletal muscle diseases characterized by congenital contractures of distal limb joints. The most common cause of DA is a mutation of the embryonic m...

Authors: Yiming Guo, William A. Kronert, Karen H. Hsu, Alice Huang, Floyd Sarsoza, Kaylyn M. Bell, Jennifer A. Suggs, Douglas M. Swank and Sanford I. Bernstein

X-linked muscular dystrophy in a Labrador Retriever strain: phenotypic and molecular characterisation

Canine models of Duchenne muscular dystrophy (DMD) are a valuable tool to evaluate potential therapies because they faithfully reproduce the human disease. Several cases of dystrophinopathies have been describ...

Authors: Inès Barthélémy, Nadège Calmels, Robert B. Weiss, Laurent Tiret, Adeline Vulin, Nicolas Wein, Cécile Peccate, Carole Drougard, Christophe Beroud, Nathalie Deburgrave, Jean-Laurent Thibaud, Catherine Escriou, Isabel Punzón, Luis Garcia, Jean-Claude Kaplan, Kevin M. Flanigan…

The ties that bind: functional clusters in limb-girdle muscular dystrophy

The limb-girdle muscular dystrophies (LGMDs) are a genetically pleiomorphic class of inherited muscle diseases that are known to share phenotypic features. Selected LGMD genetic subtypes have been studied exte...

Authors: Elisabeth R. Barton, Christina A. Pacak, Whitney L. Stoppel and Peter B. Kang

Exercise promotes satellite cell contribution to myofibers in a load-dependent manner

Satellite cells (SCs) are required for muscle repair following injury and are involved in muscle remodeling upon muscular contractions. Exercise stimulates SC accumulation and myonuclear accretion. To what ext...

Authors: Evi Masschelein, Gommaar D’Hulst, Joel Zvick, Laura Hinte, Inés Soro-Arnaiz, Tatiane Gorski, Ferdinand von Meyenn, Ori Bar-Nur and Katrien De Bock

Optimized method for extraction of exosomes from human primary muscle cells

Skeletal muscle is increasingly considered an endocrine organ secreting myokines and extracellular vesicles (exosomes and microvesicles), which can affect physiological changes with an impact on different path...

Authors: Laura Le Gall, Zamalou Gisele Ouandaogo, Ekene Anakor, Owen Connolly, Gillian Butler Browne, Jeanne Laine, William Duddy and Stephanie Duguez

A reference single-cell transcriptomic atlas of human skeletal muscle tissue reveals bifurcated muscle stem cell populations

Single-cell RNA-sequencing (scRNA-seq) facilitates the unbiased reconstruction of multicellular tissue systems in health and disease. Here, we present a curated scRNA-seq dataset of human muscle samples from 1...

Authors: Andrea J. De Micheli, Jason A. Spector, Olivier Elemento and Benjamin D. Cosgrove

Human laminin-111 and laminin-211 protein therapy prevents muscle disease progression in an immunodeficient mouse model of LAMA2-CMD

Laminin-α2-related congenital muscular dystrophy (LAMA2-CMD) is a devastating genetic disease caused by mutations in the LAMA2 gene. These mutations result in progressive muscle wasting and inflammation leadin...

Authors: Pamela Barraza-Flores, Hailey J. Hermann, Christina R. Bates, Tyler G. Allen, Timothy T. Grunert and Dean J. Burkin

Pluripotent stem cell-derived skeletal muscle fibers preferentially express myosin heavy-chain isoforms associated with slow and oxidative muscles

Skeletal muscle function is essential for health, and it depends on the proper activity of myofibers and their innervating motor neurons. Each adult muscle is composed of different types of myofibers with dist...

Authors: Tania Incitti, Alessandro Magli, Asher Jenkins, Karena Lin, Ami Yamamoto and Rita C. R. Perlingeiro

SMN-deficiency disrupts SERCA2 expression and intracellular Ca²⁺ signaling in cardiomyocytes from SMA mice and patient-derived iPSCs

Spinal muscular atrophy (SMA) is a neurodegenerative disease characterized by loss of alpha motor neurons and skeletal muscle atrophy. The disease is caused by mutations of the SMN1 gene that result in reduced fu...

Authors: Guzal Khayrullina, Kasey E. Moritz, James F. Schooley, Naheed Fatima, Coralie Viollet, Nikki M. McCormack, Jeremy T. Smyth, Martin L. Doughty, Clifton L. Dalgard, Thomas P. Flagg and Barrington G. Burnett

Exclusive vital labeling of myonuclei for studying myonuclear arrangement in mouse skeletal muscle tissue

The arrangement of myonuclei in skeletal muscle tissue has long been used as a biomarker for muscle health, but there is a dearth of in vivo exploration of potential effects of myonuclear organization on the f...

Authors: Robert Louis Hastings, Ryan T. Massopust, Seth G. Haddix, Young il Lee and Wesley J. Thompson

Functional effects of muscle PGC-1alpha in aged animals

PGC-1 (peroxisome-proliferator-activated receptor-γ coactivator-1) alpha is a potent transcriptional coactivator that coordinates the activation of numerous metabolic processes. Exercise strongly induces PGC-1...

Authors: Steven Yang, Emanuele Loro, Shogo Wada, Boa Kim, Wei-Ju Tseng, Kristina Li, Tejvir S. Khurana and Zoltan Arany

Acute conversion of patient-derived Duchenne muscular dystrophy iPSC into myotubes reveals constitutive and inducible over-activation of TGFβ-dependent pro-fibrotic signaling

In Duchenne muscular dystrophy (DMD), DYSTROPHIN deficiency exposes myofibers to repeated cycles of contraction/degeneration, ultimately leading to muscle loss and replacement by fibrotic tissue. DMD pathology...

Authors: Luca Caputo, Alice Granados, Jessica Lenzi, Alessandro Rosa, Slimane Ait-Si-Ali, Pier Lorenzo Puri and Sonia Albini

Expression of MuRF1 or MuRF2 is essential for the induction of skeletal muscle atrophy and dysfunction in a murine pulmonary hypertension model

Pulmonary hypertension leads to right ventricular heart failure and ultimately to cardiac cachexia. Cardiac cachexia induces skeletal muscles atrophy and contractile dysfunction. MAFbx and MuRF1 are two key pr...

Authors: Thanh Nguyen, T. Scott Bowen, Antje Augstein, Antje Schauer, Alexander Gasch, Axel Linke, Siegfried Labeit and Volker Adams

Angiotensin-converting-enzyme inhibitor prevents skeletal muscle fibrosis in myocardial infarction mice

Transforming growth factor beta (TGF-β)-Smad2/3 is the major signaling pathway of fibrosis, which is characterized by the excessive production and accumulation of extracellular matrix (ECM) components, includi...

Authors: Naoya Kakutani, Shingo Takada, Hideo Nambu, Junichi Matsumoto, Takaaki Furihata, Takashi Yokota, Arata Fukushima and Shintaro Kinugawa

Efficient engraftment of pluripotent stem cell-derived myogenic progenitors in a novel immunodeficient mouse model of limb girdle muscular dystrophy 2I

Defects in α-dystroglycan (DG) glycosylation characterize a group of muscular dystrophies known as dystroglycanopathies. One of the key effectors in the α-DG glycosylation pathway is the glycosyltransferase fu...

Authors: Karim Azzag, Carolina Ortiz-Cordero, Nelio A. J. Oliveira, Alessandro Magli, Sridhar Selvaraj, Sudheer Tungtur, Weston Upchurch, Paul A. Iaizzo, Qi Long Lu and Rita C. R. Perlingeiro

Correction to: Expressing a Z-disk nebulin fragment innebulin-deficient mouse muscle: effects on muscle structure and function

Following the publication of this paper [1], it was brought to the authors’ attention that one of the contributing authors was left off of the paper. The authors apologize for the unfortunate oversight. In thi...

Authors: Frank Li, Justin Kolb, Julie Crudele, Paola Tonino, Zaynab Hourani, John E. Smith III, Jeffrey S. Chamberlain and Henk Granzier

Transgenic mice expressing tunable levels of DUX4 develop characteristic facioscapulohumeral muscular dystrophy-like pathophysiology ranging in severity

All types of facioscapulohumeral muscular dystrophy (FSHD) are caused by the aberrant activation of the somatically silent DUX4 gene, the expression of which initiates a cascade of cellular events ultimately lead...

Authors: Takako I. Jones, Guo-Liang Chew, Pamela Barraza-Flores, Spencer Schreier, Monique Ramirez, Ryan D. Wuebbles, Dean J. Burkin, Robert K. Bradley and Peter L. Jones

High-throughput proteomics fiber typing (ProFiT) for comprehensive characterization of single skeletal muscle fibers

Skeletal muscles are composed of a heterogeneous collection of fiber types with different physiological adaption in response to a stimulus and disease-related conditions. Each fiber has a specific molecular ex...

Authors: Sebastian Kallabis, Lena Abraham, Stefan Müller, Verena Dzialas, Clara Türk, Janica Lea Wiederstein, Theresa Bock, Hendrik Nolte, Leonardo Nogara, Bert Blaauw, Thomas Braun and Marcus Krüger

A novel transplantable model of lung cancer-associated tissue loss and disrupted muscle regeneration

Cancer-associated muscle wasting (CAW), a symptom of cancer cachexia, is associated with approximately 20% of lung cancer deaths and remains poorly characterized on a mechanistic level. Current animal models f...

Authors: Paige C. Arneson-Wissink, Alexandra M. Ducharme and Jason D. Doles

Induction of the nicotinamide riboside kinase NAD⁺ salvage pathway in a model of sarcoplasmic reticulum dysfunction

Hexose-6-Phosphate Dehydrogenase (H6PD) is a generator of NADPH in the Endoplasmic/Sarcoplasmic Reticulum (ER/SR). Interaction of H6PD with 11β-hydroxysteroid dehydrogenase type 1 provides NADPH to support oxo...

Authors: Craig L. Doig, Agnieszka E. Zielinska, Rachel S. Fletcher, Lucy A. Oakey, Yasir S. Elhassan, Antje Garten, David Cartwright, Silke Heising, Ahmed Alsheri, David G. Watson, Cornelia Prehn, Jerzy Adamski, Daniel A. Tennant and Gareth G. Lavery

Skeletal muscle as an experimental model of choice to study tissue aging and rejuvenation

Skeletal muscle is among the most age-sensitive tissues in mammal organisms. Significant changes in its resident stem cells (i.e., satellite cells, SCs), differentiated cells (i.e., myofibers), and extracellul...

Authors: Jessy Etienne, Chao Liu, Colin M. Skinner, Michael J. Conboy and Irina M. Conboy

Mechanical factors tune the sensitivity of mdx muscle to eccentric strength loss and its protection by antioxidant and calcium modulators

Dystrophin deficiency sensitizes skeletal muscle of mice to eccentric contraction (ECC)-induced strength loss. ECC protocols distinguish dystrophin-deficient from healthy, wild type muscle, and test the effica...

Authors: Angus Lindsay, Cory W. Baumann, Robyn T. Rebbeck, Samantha L. Yuen, William M. Southern, James S. Hodges, Razvan L. Cornea, David D. Thomas, James M. Ervasti and Dawn A. Lowe

Expressing a Z-disk nebulin fragment in nebulin-deficient mouse muscle: effects on muscle structure and function

Nebulin is a critical thin filament-binding protein that spans from the Z-disk of the skeletal muscle sarcomere to near the pointed end of the thin filament. Its massive size and actin-binding property allows ...

Authors: Frank Li, Justin Kolb, Julie Crudele, Paola Tonino, Zaynab Hourani, John E. Smith III, Jeffrey S. Chamberlain and Henk Granzier